anaerobic workstation

anaerobic workstation with hypoxic function for inoculation

The anaerobic hypoxic workstation creates a hypoxic or anaerobic environment similar to that of human tissue or natural ecosystem by strictly regulating the gas composition and humidity in the chamber to meet the demand for special atmosphere in the fields of microbiology, clinical testing, food fermentation and so on.

Description

Anaerobic Hypoxic Workstation

The anaerobic hypoxic workstation supports simultaneous cultivation of multiple strains of bacteria, facilitating scientific research and industrial applications.

Features

  1. Accurate gas regulation: Precisely adjusts oxygen and carbon dioxide concentrations, simulating low-oxygen states for microaerophilic and anaerobic bacteria growth.
  2. Automatic humidity control: Built-in system dynamically manages chamber humidity, preventing media from becoming too dry or too wet.
  3. Flexible operation mode: Supports free switching between anaerobic and low oxygen modes to suit experimental needs.
  4. Various operating interfaces: Provides sleeveless and traditional sleeve operation for different lab habits and safety requirements.
  5. High-efficiency air filtration: Optional HEPA system prevents airborne particles and impurities from entering the chamber.

Core Advantages

  1. Multi-species applicability: Supports national standard microbial culture needs, including Shigella (anaerobic, 41.5°C), Campylobacter jejuni (aerobic, 25°C), Clostridium perfringens (anaerobic, 36°C), Clostridium botulinum (anaerobic, 35°C), and Lactobacillus (anaerobic, 36°C).
  2. Stable atmosphere: Catalyst and regulator maintain oxygen content within a reasonable range for reliable microorganism growth.
  3. Safe, convenient operation: Sleeveless system reduces glove breakage risk, improves flexibility, and operator safety.
  4. Energy saving, eco-friendly: Low power consumption with intelligent control for gas and humidity allocation.

Working Principle

  1. Gas replacement: Built-in vacuum pump removes air, then fills the chamber with preset nitrogen, hydrogen, and CO₂ mixture for low-oxygen or anaerobic environments.
  2. Catalytic deoxidization: Hydrogen and residual oxygen react with palladium catalyst, forming water and reducing oxygen concentration.
  3. Automatic humidity adjustment: Humidification device and sensor monitor and maintain optimal humidity.
  4. Gas monitoring and feedback: Sensors detect O₂ and CO₂ in real time, software adjusts input for stable parameter control.

Application Fields

  1. Microbiology research: Cultivation of pathogenic, probiotic, and environmental bacteria needing special oxygen conditions.
  2. Clinical testing: Accurate culture for pathogen identification, drug sensitivity, and medical diagnostics.
  3. Food & fermentation industry: Efficient growth of probiotics and fermentation strains, process optimization, and quality improvement.
  4. Environmental science & ecology: Simulates low-oxygen soil/sediment conditions for ecological studies.
  5. Drug development & toxicology: Simulates body’s low oxygen environment to evaluate drug effects and advance precision medicine.

Technical Parameters & Configuration List

  • Power supply: 220V, 50/60Hz
  • Power: ~4A
  • Ambient temperature: 25°C
  • Relative humidity: <90%
  • Configuration includes:
    • 1 main unit
    • 1 set sleeveless in/out system
    • 8 Petri dish racks
    • 1 fluorescent lamp
    • 1 UV lamp
    • 1 bag catalyst (600g)
    • 1 bag decontamination (600g)
    • 1 bottle lubrication powder
    • 1 set anaerobic indicator pump (A/B liquid)
    • 1 set single-dish transfer system
    • 1 internal power socket
    • 1 power cord
    • 1 operation manual
    • 1 set installation & debugging tools
Technical Parameters

Model: AH50

  1. Fast Transfer Gate: Built-in, 12L capacity; transfers 40 × 90mm dishes at once; compatible with triangular vials and homogenization bags (height <185mm). N₂ blowing wash completes transfer in 60 seconds, in or out of the workstation.
  2. Mechanical Press Door Lock: Ensures airtightness during transfer chamber rinsing, preventing air penetration.
  3. One-Handed Operation: Inner door of the transfer gate can be opened/closed easily with one hand.
  4. Large Capacity: Holds 760 × 90mm dishes; >80 × 250mL triangular bottles; >50 × 500mL triangular bottles; >100 × 250mL reagent bottles; >80 × 500mL reagent bottles; >100 homogenizing bags.
  5. Removable Side Panels: Allows access for small equipment and thorough box cleaning. Use special disinfectant (tetrasodium EDTA + 3-tert-butylphenol sodium lauryl polyoxyethylene ether sulfate) for effective, non-damaging cleaning.
  6. Gas Modes:
    • Anaerobic mode: Double gas—one bottle anaerobic mixture (80% N₂ + 10% CO₂ + 10% H₂), one bottle nitrogen.
    • Low oxygen mode: Three gas—one bottle CO₂, one bottle nitrogen, one bottle compressed air.
  7. 7-inch Touch Screen: For parameter setting, status monitoring, and alarm display.
  8. Humidity Control: Fully automatic system, 0–85% (high humidity up to 90%); optional ultrasonic humidification. Intelligent semiconductor dehumidification device.
  9. Lighting: Standard fluorescent lamp and 254nm UV sterilization lamp.
  10. Optional HEPA Filtration: Achieves up to Class 100 clean environment.
  11. Anaerobic Indication: Standard indication system (liquid & pump); optional digital indicator (0–3000ppm).
  12. Oxygen Control Range: Anaerobic mode <5ppm; low oxygen mode 0–25% (0.1% increments).
  13. CO₂ Control Range: 0–20% (0.1% increments).
  14. Humidity Control Range: 0–85%.
  15. Temperature Control: Room temp +3°C to 52°C; display accuracy 0.1°C; uniformity ±0.3°C; optional small incubator for higher temperature cultures.
  16. Sleeveless Fast Operating System: Supports bare hand operation without vacuum nitrogen filling; optional traditional sleeve operation.
  17. Built-in Power Socket: Standard configuration.
  18. Equipment Dimensions: 1280mm (L) × 730mm (H) × 680mm (D).
  19. USB Data Download: Data can be exported via USB.
  20. Automatic Gas Overcurrent Protection: Shuts inlet solenoid valve after 5+ minutes of continuous gas inlet.