anaerobic-hypoxic workstation

anaerobic-hypoxic workstation with timed air inlet function

The anaerobic and hypoxic workstation provides a controlled hypoxic and even anaerobic environment for microbiology, cell biology, molecular medicine and other research.

Description

Anaerobic Hypoxia Workstation

Enables researchers to culture, manipulate, and analyze anaerobic bacteria, microaerobic bacteria, or hypoxia-sensitive cells under conditions close to in vivo physiological environments. Suitable for experiments simulating tissue hypoxia, tumor microenvironment, inflammatory response, and other complex physiological states.

Main Features

  1. Precise gas control: Oxygen concentration adjustable to less than 0.1%, supports long-term low-oxygen/no oxygen conditions.
  2. CO₂ concentration regulation: Built-in high-sensitivity sensor, automated concentration management for cell metabolism.
  3. Automatic humidity regulation: Ultrasonic humidification with feedback system maintains constant humidity and prevents medium evaporation.
  4. High-efficiency filtration: Built-in HEPA-U16 grade filter ensures clean internal air, prevents sample contamination.
  5. Humanized operation: Flexible, fully sealed sleeve for long-term, pollution-free, safe and efficient operation.
  6. Timed air intake: Programmable gas supply mechanism supports multi-stage oxygen pressure change experiments.
  7. Multiple lighting systems: Equipped with fluorescent and UV sterilization lamps for observation and hygiene control.
  8. Energy-saving, stable design: 6A low-power operation; power socket supports parallel use of multiple instruments.

Advantages

  1. Simulate real physiological environment: Can create complex oxygen conditions like tumor hypoxia, inflammatory tissue, intestinal environment.
  2. Save culture time: Continuous, stable low oxygen control avoids contamination and failure from repeated transfer.
  3. Strong versatility: Supports culture of microorganisms, mammalian cells, stem cells, etc.
  4. Modular design: Supports expansion for different gas ports, culture apparatus, data acquisition modules.
  5. High operating comfort: Ergonomically optimized cuff and layout, no fatigue during long-term operation.

Working Principle

The workstation mixes high-purity nitrogen, oxygen, and carbon dioxide in set ratios via a multi-way gas mixing system and delivers them to a closed culture chamber. High-precision oxygen/carbon dioxide sensors provide real-time monitoring and feedback control for linear oxygen concentration adjustment (normoxia to hypoxia, even 0%). The humidification system maintains constant humidity; HEPA filtration ensures clean, dust-free air. Experimenters operate aseptically with sealed cuffs; external control enables automatic and programmable management.

Application Areas

  1. Microbiology research: Cultivation, isolation, and research of strict anaerobes (e.g., Clostridium spp., Bacteroides spp.)
  2. Tumor biology: Study tumor metabolism, proliferation, invasion, etc., under low oxygen.
  3. Cardiovascular research: Ischemia/reperfusion models, intermittent hypoxia, etc.
  4. Stem cell research: Simulate hypoxic environments favorable for proliferation.
  5. Drug screening & toxicology: Detect drug activity and toxicity in low oxygen.
  6. Infection & immunology: Study pathogen-host interactions in low oxygen.
Technical Parameters

Model: AH60

  1. Quick Transfer Gate: 16L capacity, transfers 60 × 90mm dishes at once; N₂ purge completes transfer in 60 seconds, in or out of workstation.
  2. Intelligent Protection: Transfer gate ensures internal environment is unaffected during transfer.
  3. Easy Operation: One-handed, single-key open/close of inner transfer gate door.
  4. Large Capacity: Holds 900 × 90mm dishes, >100 × 250mL triangular bottles, >60 × 500mL triangular bottles, >120 × 250mL reagent bottles, >100 × 500mL reagent bottles, >120 homogenizing bags.
  5. Removable Side Panels: Allows access for equipment and thorough cleaning.
  6. Gas Modes:
    • Anaerobic: Double gas—one bottle anaerobic mixture (80% N₂ + 10% CO₂ + 10% H₂), one bottle nitrogen.
    • Low oxygen: Three gas—CO₂, nitrogen, compressed air.
  7. Touch Screen: 7-inch color screen for parameter setting, status monitoring, and alarm display.
  8. Humidity Control: Ultrasonic humidification and automatic system; range: ambient to 90%; low water level auto-stop function.
  9. Lighting: Standard fluorescent and 254nm UV sterilization lamps.
  10. HEPA Filter: Built-in U16 filter, filtration efficiency 99.99995%@0.12μm; meets ISO14644-1 class 3 standard.
  11. O₂ & CO₂ Control: Oxygen: anaerobic mode <5ppm (actual test images provided); low oxygen mode 0–25% (0.1% increments). CO₂: 0–20% (0.1% increments). Sensors feature automatic calibration.
  12. Gas Cycle Function: 6-segment O₂/CO₂ cycling; set target value, time, cycles; supports hypoxia, fluctuation, intermittent, and high oxygen experiments; data downloadable via USB.
  13. Temperature Control: Room temp +3°C to 52°C; display accuracy 0.1°C; uniformity ±0.3°C.
  14. Long Sleeve Operation: Built-in, fully sealed sleeve system ensures zero oxygen entry; no vacuum pump needed, avoids cumbersome operation.
  15. Power Sockets: 3 built-in sockets standard.
  16. Data Logging: USB interface; records O₂, CO₂, temperature, humidity, air intake time; up to 30 days.
  17. Alarm System: High/low temp, humidity out of range, gas mixing, nitrogen/CO₂/compressed air low pressure, leakage alarms; automatic gas overcurrent protection (shuts solenoid after 5 min continuous air intake).
  18. Timed Air Intake: Intake time arbitrarily set/displayed on touchscreen; fast mode supported.
  19. Palladium Catalyst: High efficiency, no frequent activation, lasts 4 years.
  20. Dimensions: 1680mm (L) × 730mm (H) × 680mm (D).