bottom-driven single-use bioreactor with low-shear agitation

Bottom-Driven Single-Use Bioreactor

The axial flow-dominated, angled-in stirring paddles of the bottom-driven single-use bioreactor not only retain the mixed culture effect of a traditional reactor, but also offer the advantages of more efficient production performance and avoidance of cleaning and sterilization validation. It is widely used for cell growth, cell viability maintenance and metabolic process optimization, and is suitable for all kinds of scale culture from 50L to 2000L.

Features

• Good axial flow mixing and substance transfer: The down-mixing design enables excellent axial flow, ensures uniform mixing of culture solution in the reactor, and optimizes the delivery of oxygen and nutrients. Compared with the traditional reactor, the material transfer is more efficient and the cell culture environment is more uniform.
• High-quality parts, system stability: The reactor adopts high-quality components to ensure the stability and durability of the system, reducing the risk of equipment failure and providing more reliable operation guarantee.
• Modular design, support for customization: Modular design makes the equipment more flexible, customers can be customized according to the actual needs, adapt to the requirements of different scales and processes to meet individual needs.
• Pressure control function, provide security protection: The equipment is equipped with advanced pressure control function, can real-time monitor and adjust the pressure inside the reactor, to ensure that the equipment is in a safe and stable state during the working process, effectively preventing pressure abnormalities on the culture environment.
• Three levels of authority, audit trail and electronic data reports: The system supports three levels of authority settings to ensure the safety and standardization of operations. At the same time, the device is able to generate complete electronic data reports for subsequent storage, transmission and audit trail.
• Touch-screen software control, simple and intuitive operation: Equipped with efficient touch-screen control interface, the software is feature-rich and easy to operate, providing a clear feedback system and logic to ensure that the operator can quickly grasp and efficient operation.
• Flexible ventilation design: Adopting large-bubble and micro-bubble ventilation system, together with the design of disposable reaction bag chassis, it can flexibly adjust the efficiency of gas transmission and adapt to the needs of different types of cell culture.
• Perfect FAT, SAT, IQ, OQ documents and services: Provide complete factory acceptance test, on-site acceptance test, installation confirmation and operation confirmation documents and services to ensure that the equipment meets the GMP production standards.

Working Principle

1. The bottom-driven single-use bioreactor optimizes the material transfer and gas-liquid mixing inside the reactor through the down-mixing stirring design, mainly through the mixing effect of axial flow and slanting into the stirring paddles. The bubbles inside the equipment can be aerated through the form of large bubbles and microbubbles, which effectively improves the oxygen dissolution efficiency and ensures sufficient oxygen supply to the cells in suspension cell culture. The pressure control system can monitor the internal pressure of the reactor in real time to ensure the stable operation of the bioreactor.
2. In addition, the modular design of the system supports different scales of cultivation needs, and users can adjust different reactor configurations and operating parameters according to the requirements of experiments or production to realize the cultivation effect. The touch screen operation interface is simple and intuitive, users can easily manage the culture process by setting and adjusting parameters.

Application Areas

• Suspension cell culture: Suitable for large-scale suspension cell culture, widely used in pharmaceuticals, vaccine production and other fields to ensure the optimization of cell growth and metabolic processes.
• Adherent cell microcarrier culture: Can support the culture of adherent cells on microcarriers, commonly used in stem cell research, gene therapy and cell therapy applications.
• Process development: In the process development stage, the reactor is capable of conducting experiments under different conditions to help research development personnel optimize the culture process and improve the yield and quality of products.
• Process scale-up: From laboratory scale to pilot scale process scale-up, the reactor provides stable conditions to ensure the consistency of reaction and product quality during the scale-up process.
• Pilot: It is applicable to the pilot stage, helping enterprises to carry out small-scale validation before production amplification, providing data support and technical guarantee for the subsequent industrialized production.
• Seed amplification: It can be used for cell seed amplification to provide sufficient cell number for subsequent large-scale cell culture.
• GMP production: Used in a production environment that meets GMP standards, suitable for the biopharmaceutical industry, cell therapy production and other areas that require strict quality control and equipment standards.