anaerobic hypoxic workstation

anaerobic hypoxic workstation with only one working window

Anaerobic hypoxic workstation is a kind of experimental equipment designed for microbial research and cell culture, capable of simulating anaerobic and hypoxic conditions in a highly controlled environment, which is widely used in experiments in the fields of bacteriology and cytology.

Description

Anaerobic Hypoxia Workstation

The Anaerobic Hypoxia Workstation provides a growth environment for microorganisms, anaerobes, microaerobes, and cells by independently controlling oxygen and carbon dioxide concentrations and humidity.

Features

  1. Precise Oxygen Control: Adjustable oxygen concentration simulates the human body’s low oxygen environment, suitable for microaerobe and anaerobe cultivation.
  2. CO₂ Concentration Control: Precise regulation optimizes cell culture environment and improves results.
  3. Automatic Humidity Regulation: Intelligent humidity control system maintains optimal humidity, avoiding dry or overly wet conditions.
  4. High-Efficiency Filtration: Built-in HEPA U16 filter removes fine particles, ensures clean air and prevents external contamination.
  5. Long Sleeve Operating System: User-friendly design facilitates easy operation and effectively prevents contamination.
  6. Flexible Air Intake Timing: Set air intake time and frequency to adapt to different experimental needs.
  7. Single Working Window: Reduces outside air interference, ensures experimental environment stability.

Advantages

  1. Precise Environmental Control: Simultaneous control of oxygen, CO₂, and humidity for stable experiments.
  2. High-Efficiency Filtration: HEPA system and closed design prevent external pollution of cultures.
  3. Versatile Experimental Adaptability: Suitable for anaerobic/microaerobic bacteria and cell experiments including hypoxia and oxygen fluctuation.
  4. Easy, Safe Operation: Built-in long sleeve system improves convenience and safety.

Working Principle

The workstation simulates in vivo microenvironments by real-time monitoring and adjustment of internal gases. Oxygen and CO₂ sensors continuously collect data; an automatic control system regulates gas concentrations to maintain preset conditions. Ultrasonic humidification stabilizes humidity. HEPA U16 filter removes airborne contaminants.

Areas of Application

  1. Microbiology research: Culturing anaerobic and microaerobic bacteria, oxygen-sensitive species.
  2. Cell culture: Stable low oxygen/CO₂ environment for cancer cells, stem cells, etc.
  3. Biomedical research: Cell experiments under hypoxia and oxygen fluctuation for studying cell behaviors.
  4. Environmental simulation: Mimicking low oxygen/hypoxia for physiological and pathology studies.
  5. Drug research: Simulating low oxygen for drug screening and pharmacology.
  6. Food & environmental testing: Microbial and environmental sample culturing for monitoring and analysis.

Main Configuration

  • Host: 1 unit
  • 4 sets fully sealed sleeve inlet/outlet system
  • HEPA U16 filter: 1 set
  • Oxygen sensor: 1 set
  • CO₂ sensor: 1 set
  • Multi-way gas mixer: 1 set
  • Gradient circulation control system: 1 set
  • Ultrasonic humidification system: 1 set
  • 16 Petri dish racks
  • Fluorescent lamp: 1 pc
  • UV lamp: 1 pc
  • Catalyst (200g): 5 bags
  • Regulator (200g): 5 bags
  • Lubricating powder: 1 bottle
  • Internal power sockets: 4 pcs
  • Power cord: 1 pc
  • Operation manual: 1 set
  • Installation & commissioning accessories: 1 set
Technical Parameters

Model: AH80

  1. Quick Transfer Gate: 20L capacity, transfers 60 × 90mm dishes at once; N₂ purge completes transfer in 60 seconds, in or out of workstation.
  2. Intelligent Protection: Transfer gate ensures the internal environment remains unaffected during transfers.
  3. Easy Operation: One-handed, single-key open/close of inner transfer gate door.
  4. Large Capacity: Holds 1200 × 90mm dishes, >120 × 250mL triangular bottles, >80 × 500mL triangular bottles, >160 × 250mL reagent bottles, >120 × 500mL reagent bottles, >160 homogenizing bags.
  5. Removable Side Panels: Allows access for equipment and thorough cleaning.
  6. Gas Modes:
    • Anaerobic: Double gas—one bottle anaerobic mixture (80% N₂ + 10% CO₂ + 10% H₂), one bottle nitrogen.
    • Low oxygen: Three gas—CO₂, nitrogen, compressed air.
  7. Touch Screen: 7-inch color screen for parameter setting, status monitoring, and alarm display.
  8. Humidity Control: Ultrasonic humidification and automatic system; range: ambient to 90%; low water level auto-stop function.
  9. Lighting: Standard fluorescent and 254nm UV sterilization lamps.
  10. HEPA Filter: Built-in U16 filter, filtration efficiency 99.99995%@0.12μm; meets ISO14644-1 class 3 standard.
  11. O₂ & CO₂ Control: Oxygen: anaerobic mode <5ppm (actual test images available); low oxygen mode 0–25% (0.1% increments). CO₂: 0–20% (0.1% increments). Sensors feature automatic calibration.
  12. Gas Cycle Function: 6-segment O₂/CO₂ cycling; set target value, time, cycles; supports hypoxia, fluctuation, intermittent, and high oxygen experiments; data downloadable via USB.
  13. Temperature Control: Room temp +3°C to 52°C; display accuracy 0.1°C; uniformity ±0.3°C.
  14. Long Sleeve Operation: Built-in, fully sealed sleeve system ensures zero oxygen entry; no vacuum pump needed, avoiding cumbersome operation.
  15. Power Sockets: 4 built-in sockets standard.
  16. Data Logging: USB interface; records O₂, CO₂, temperature, humidity, air intake time; up to 30 days.
  17. Alarm System: High/low temp, humidity out of range, gas mixing, nitrogen/CO₂/compressed air low pressure, leakage alarms; automatic gas overcurrent protection (shuts solenoid after 5 min continuous air intake).
  18. Timed Air Intake: Intake time arbitrarily set/displayed on touchscreen; fast mode supported.
  19. Palladium Catalyst: High efficiency, no frequent activation, lasts 4 years.
  20. Equipment Size: 2230mm (L) × 830mm (H) × 800mm (D).