anaerobic hypoxic workstation

anaerobic hypoxic workstation with two gloves for use

The anaerobic hypoxia workstation creates an anaerobic or microaerobic state by accurately adjusting the oxygen and carbon dioxide concentration in the chamber, which is suitable for the cultivation, transgeneration and observation of a variety of microorganisms.

Description

Anaerobic Hypoxia Workstation Overview

The anaerobic hypoxia workstation meets the needs of national standard microorganisms such as Shigella, Campylobacter jejuni, Clostridium perfringens, Clostridium botulinum, and Lactobacillus in different temperature and gas environments. It is widely used in scientific research, clinical, and industrial testing.

Main Features

  1. Precise oxygen concentration control: Built-in highly sensitive sensors, real-time monitoring and automatic adjustment, replicating low oxygen states.
  2. CO₂ concentration regulation: Precise gas mixing, optimizing the cellular respiratory environment for healthy growth.
  3. Intelligent humidity management: Fully automatic humidity control to prevent cultures from drying or over-wetting.
  4. Various operation modes: Supports sleeveless and traditional sleeve operation for flexibility and safety.
  5. Multi-mode environment switching: Flexibly switch between anaerobic and low-oxygen modes as needed.
  6. High-efficiency clean filtration: Optional HEPA device to prevent external pollutants.
  7. Double glove design: Equipped with two gloves for efficient two-handed operation.

Working Conditions

  • Power: 220V, 50/60Hz, 4A
  • Environment: Ambient temperature 25°C, humidity <90%

Advantages

  1. Strong environmental stability: Advanced gas regulation and catalytic system ensures long-term stable anaerobic/low-oxygen environment.
  2. Wide strain applicability: Suitable for diverse microorganisms, including clinical pathogens and industrial fermentation strains.
  3. Safe and efficient operation: Sleeveless system reduces leakage risk, improves safety and flexibility.
  4. Energy saving and environmental protection: Intelligent gas and humidity control reduces consumption and increases efficiency.

Working Principle

  1. Gas replacement: Vacuum pumps remove air, then inject nitrogen, hydrogen, and carbon dioxide as needed to quickly establish desired environment.
  2. Catalytic deoxygenation: Hydrogen reacts with residual oxygen under catalyst, producing water and further reducing oxygen.
  3. Automatic humidity control: Built-in humidity sensor and device for real-time detection and adjustment.
  4. Gas concentration feedback: Sensors continuously monitor concentrations; control system dynamically adjusts mixture for stability.

Application Fields

  1. Microbial basic research: Supports culture, isolation, metabolic and antibiotic sensitivity testing of various microorganisms.
  2. Clinical pathogen detection: Cultures human pathogens for reliable diagnosis.
  3. Biopharmaceutical and fermentation: Optimizes fermentation strain growth, enhancing drug and product yield/quality.
  4. Food safety testing: Cultivates harmful anaerobic and microaerobic bacteria to ensure food hygiene.
  5. Environmental microbiology: Simulates low-oxygen ecosystems for studying microbial ecology in soil, water, etc.
Technical Parameters

Model: AH50G

  1. Fast transfer gate: 12L capacity; transfers 40 × 90mm dishes at once; N₂ blowing wash; 60 seconds to complete sample/equipment transfer in or out of workstation.
  2. Electric pneumatic door lock: Inner and outer doors intelligently interlocked; prevents air penetration during transfer chamber rinsing.
  3. One-handed operation: Single-key, easy opening and closing of inner door.
  4. Large capacity: Holds 850 × 90mm dishes; >90 × 250mL triangular vials; >60 × 500mL triangular vials; >110 × 250mL reagent vials; >90 × 500mL reagent vials; >110 homogenization bags.
  5. Removable side panels: Easy access for small equipment and thorough cleaning.
  6. Gas supply: Single gas (80% N₂ + 10% H₂ + 10% CO₂) or double gas (pure nitrogen + oxygen-free mixture).
  7. Touch screen: 7-inch color screen for parameter setting, status monitoring, and alarm display.
  8. Ultrasonic humidification: Fully automatic system with low water level auto stop; humidity control: ambient to 99%.
  9. Lighting: Standard LED lighting and 254nm UV sterilization lamp.
  10. HEPA filter (optional): Built-in U16 filter; ISO14644-1 class 3 standard filtration.
  11. Oxygen control: Anaerobic mode <50ppm; low oxygen mode 0–25%, adjustable at 0.1% increments.
  12. CO₂ control: 0–20%, adjustable at 0.1% increments.
  13. Gas cycling function: 6-section O₂/CO₂ cycle; set target concentration, time, cycles; supports paroxysmal hypoxia, high/low oxygen fluctuation, intermittent hypoxia, etc.; USB data download.
  14. Temperature control: Room temp +3°C to 52°C; accuracy 0.1°C; uniformity ±0.3°C.
  15. Bare hand operation: Long sleeve system; vacuum + nitrogen process ensures 100% oxygen removal when entering workstation.
  16. Foot switch control: Vacuum and nitrogen filling via foot switch; standard external vacuum pump.
  17. Built-in power sockets: 2 standard sockets.
  18. Data logging: USB interface; records O₂, CO₂, temperature, humidity, air intake time; up to 30 days.
  19. Alarm system: High/low temp, humidity out of range, gas mixing, nitrogen/CO₂/compressed air low pressure, leakage alarms; automatic gas overcurrent protection (shuts inlet solenoid after 5 min continuous air intake).
  20. Timed air intake system: Intake time arbitrarily set and displayed on touchscreen; fast mode also supported.
  21. Palladium catalyst: High efficiency; 4+ years use; no frequent activation needed.
  22. Dimensions: 1405mm (L) × 740mm (H) × 720mm (D).