anaerobic microaerobic workstation with uv sterilizing lamp

Anaerobic microaerobic workstation is a high-precision microbial culture equipment specialized in constructing and maintaining low-oxygen and even anaerobic experimental environments, which is widely used in the study of bacterial groups with obvious differences in aerobicity.

Description

Anaerobic Microaerobic Workstation

The closed operating system of the anaerobic microaerobic workstation enables precise regulation of key parameters such as gas concentration, temperature, and humidity, providing a constant and stable hypoxic or anaerobic environment for operations like inoculation, passaging, media conversion, and colony observation. It is ideal for strains strictly reliant on anaerobic conditions or sensitive to oxygen concentration, such as Shigella, Campylobacter jejuni, and Clostridium botulinum.

Main Features

  1. Multi-species adaptation: Temperature and gas environment can be set for various strains, meeting national standards for cultures like Shigella, Campylobacter, and Clostridium.
  2. Precise environmental control: Oxygen concentration adjustable from 0% to 10%, supporting complete anaerobic to microaerobic experimental needs.
  3. Adjustable temperature control: Precision thermostat module allows culture temperature settings from 25°C to 45°C.
  4. Integrated operation chamber: Fully enclosed area with transparent walls and sealed sleeves for sterile, anaerobic operation.
  5. Intelligent monitoring: Real-time display and recording of gas concentration, temperature, humidity, with abnormal alarm functions.
  6. Convenient gas channel: Multiple gas interfaces support precise mixing and injection of nitrogen, hydrogen, carbon dioxide, etc.
  7. Clean air management: HEPA high-efficiency filtration device prevents sample contamination and cross-infection.

Advantages

  1. High operational flexibility: Supports full anaerobic operation and precise micro-oxygen control (1–5%) for varied experimental design.
  2. Improved repeatability & safety: Automated environment control and monitoring reduce human error and enhance experimental consistency.
  3. Safer sample processing: Avoids oxygen exposure risk from repeated tank switching, maintains microbial activity.
  4. Energy-saving & efficient: Low power consumption (4A) for stable operation.
  5. High-throughput operation: Supports multiple media and sample processing for medium and large labs/testing centers.

Working Principle

  1. Gas mixing & injection: High-precision mixing of nitrogen, hydrogen, and CO₂, injected into the chamber.
  2. Chemical deoxidation: Built-in catalyst and trace hydrogen react to rapidly consume residual oxygen.
  3. Constant temperature & humidity: Heating element and ultrasonic humidifier maintain stable environmental conditions.
  4. Sealed operation: Cuffs and hatches prevent oxygen leakage, keeping samples in target gas state.
  5. Feedback control: Sensors monitor O₂ and CO₂, automatically adjusting gas input for stability.

Application Fields

  1. Public health & disease control: Quantitative analysis of pathogenic bacteria for food safety standards (e.g., Shigella, Clostridium perfringens).
  2. Clinical microbiology: Cultivation and identification of anaerobic pathogens (e.g., Clostridium botulinum, Clostridium difficile).
  3. Food microbiology: Culture/ID of strains related to food preservation (e.g., fermented lactic acid bacteria).
  4. Environmental microecology: Studies on anaerobic ecosystems, soil, water, sewage flora metabolism.
  5. Basic scientific research: Cell metabolism, virulence expression, drug resistance mutation under microaerobic conditions.
  6. Biopharmaceutical & vaccine R&D: Stable cultivation environment for anaerobic strains and metabolite collection.
Technical Parameters

Model: AM50

  1. Quick Transfer Gate: 23L capacity; transfers 60 × 90mm dishes at once. Internal size: 290 × 250 × 320mm; door size: 250 × 250mm. N₂ blowing enables smooth transfer in 60 seconds.
  2. Intelligent Protection: Transfer gate ensures the internal environment remains unaffected during sample/equipment transfer.
  3. Easy Operation: One-handed, single-key open/close for the inner transfer gate door.
  4. Large Capacity: Holds 900 × 90mm flat dishes.
  5. Front Panel Access & Cleaning: Removable front panel (opening: 74cm × 47cm, depth: 65cm) for equipment/cage access and cleaning. Use special disinfectant (tetrasodium EDTA + 3-tert-butylphenol lauryl polyoxyethylene ether sodium sulfate) for safe, effective cleaning.
  6. Gas Modes:
    • Anaerobic: Double gas—one bottle of anaerobic mixture (80% N₂ + 10% CO₂ + 10% H₂), one bottle nitrogen.
    • Low oxygen: Three gas—CO₂, nitrogen, compressed air (miniature system included, no cylinder needed).
  7. Touch Screen: 7-inch color screen for parameter setting, status monitoring, and alarms.
  8. Humidity Control: Ultrasonic humidification and automatic system; range: ambient to 90%; low water level auto-stop function.
  9. Lighting: Standard fluorescent and 254nm UV sterilization lamps.
  10. HEPA Filtration (optional): Built-in U16 filter; ISO14644-1 class 3 standard filtration efficiency.
  11. O₂ & CO₂ Control: Oxygen: anaerobic mode <5ppm; low oxygen mode 0–25% (0.1% increments). CO₂: 0–20% (0.1% increments). Sensors feature automatic calibration.
  12. Gas Cycle Function: 6-segment O₂/CO₂ cycling; programmable target values, time, cycles. Supports hypoxia, fluctuation, intermittent, high oxygen, and other experiments; data downloadable via USB.
  13. Temperature Control: Room temp +3°C to 52°C; display accuracy 0.1°C; uniformity ±0.3°C.
  14. Long Sleeve Operation: Built-in, fully sealed sleeve system ensures zero oxygen entry; no vacuum pump needed, avoids cumbersome operation.
  15. Power Sockets: 2 built-in waterproof sockets (standard).
  16. Data Logging: USB interface; records O₂, CO₂, temperature, humidity, air intake time; up to 30 days.
  17. Alarm System: High/low temp, humidity out of range, gas mixing, nitrogen/CO₂/compressed air low pressure, gas leakage alarms; automatic gas overcurrent protection (shuts solenoid after 5 min continuous air intake).
  18. Timed Air Intake: Programmable air intake time and fast mode; settings displayed on touchscreen.
  19. Palladium Catalyst: High efficiency, no frequent activation, lasts 4 years.
  20. Equipment Size: 1380mm (L) × 830mm (H) × 800mm (D).