real-time fluorescence qpcr with 12-column gradient function

Real-time Fluorescence Quantitative PCR Instrument

The real-time fluorescence qPCR instrument is equipped with a large touch screen and powerful software, designed to meet the diverse needs of scientific research and clinical applications in real-time fluorescence quantitative PCR.

Main Features

1. Fast & Accurate Temperature Control: Semiconductor system supports temperature changes at 6 °C/sec; cycle life up to 1 million times—stable and durable.
2. Top Fluorescence Detection: Top excitation and detection compatible with white PCR tubes; effectively shields background light for improved sensitivity and signal-to-noise ratio.
3. Synchronized Multi-well Acquisition: Simultaneous fluorescence signal collection from all wells in the same channel; eliminates delay errors from sequential detection.
4. Large Full-color Touch Screen: 10-inch HD true-color touch screen enables independent running, monitoring, and analysis without a computer.
5. Advanced CCD Imaging: SSLP short-range static CCD imaging provides high signal stability and avoids fiber clogging/wear.
6. Maintenance-free LED Light Source: Long-life LEDs require no frequent replacement or calibration; stable, reliable detection.
7. Gradient Temperature Optimization: 12-column gradient function streamlines amplification condition screening.
8. Powerful Analysis Software: Built-in qPCR analysis software supports remote operation/data analysis; free lifetime upgrades.
9. Centralized Multi-device Control: Optional software enables one computer to manage multiple PCR instruments.
10. Large-capacity Program Storage: Stores up to 15,000 programs; unlimited import via USB drive.
11. Multi-interface Support: Ethernet and USB2.0 interfaces for easy data export and remote management.

Advantages

1. Fast Reaction & High Efficiency: Rapid temperature cycling shortens PCR cycles and increases throughput.
2. Sensitive & Stable Detection: Static CCD imaging and top detection enhance signal quality and reduce errors.
3. Convenient Independent Operation: Large touch screen for stand-alone use in varied environments.
4. Low Maintenance Costs: Maintenance-free LED and non-mechanical optics reduce upkeep and cost.
5. Intelligent Data Management: Extensive program storage and remote management for efficient lab operations.

Working Principle

Utilizes high-efficiency semiconductor Peltier elements for precise temperature control, with multi-point sensors for real-time feedback. Top fluorescence excitation and static CCD imaging enable simultaneous signal capture from all wells, avoiding mechanical delay and optical errors. Long-life LED provides stable excitation; built-in software analyzes data, generates amplification curves, and calculates quantitative results for accurate gene detection.

Application Areas

1. Clinical Medicine: Pathogen detection, genetic disease diagnosis, tumor marker monitoring.
2. Basic Research: Gene expression analysis, functional gene validation, transgenic research.
3. Environmental Monitoring: Microorganism quantification in water and soil.
4. Food Safety: Pathogen and GMO component detection.
5. Forensic Science: DNA quantification, paternity testing.
6. Drug Development: Gene expression/mutation analysis of drug targets.
7. Education & Training: PCR and molecular biology teaching.