three-block real-time fluorescence quantitative pcr

The three-slot real-time fluorescence quantitative PCR instrument is a highly integrated molecular testing equipment, equipped with three independently operated 32-well modules, which can be used to carry out three different experiments at the same time and improve the efficiency of laboratory work.

Description

Three-slot Real-time Fluorescence Quantitative PCR Instrument

This advanced qPCR instrument is ideal for clinical diagnosis, scientific research, and teaching, providing high flexibility and efficiency for a wide range of applications.

Main Features

  1. Three Independent Modules: Three groups of 32-well modules, each supporting separate programs for true multi-tasking—one machine, three uses.
  2. Highly Flexible Channel Configuration: Channel filters are freely matched and expandable to 21 channels for diverse experimental needs.
  3. Overhead Fluorescence Detection (T-Optical): Optimized for white PCR tubes; shields background interference to enhance signal sensitivity and signal-to-noise ratio.
  4. Synchronous Fluorescence Acquisition: All wells acquire signals simultaneously, eliminating time resolution errors.
  5. Adjustable Display: Flexible screen angle for improved comfort and visibility.
  6. Multiple Data Analysis Modes: Supports absolute/relative quantification, genotyping, positive/negative determination, melting curve analysis, optional protein melting, and high-resolution melting (HRM) analysis.
  7. Advanced Optical Imaging: SSLPI static fluorescence imaging with a compact, stable system for stronger signals.
  8. Power Failure Protection & Stand-alone Operation: Ensures experimental safety and continuity, supports offline independent operation without a computer.

Advantages

  1. Efficient Multi-tasking: Independent modules boost sample throughput and experimental flexibility.
  2. Multi-channel Support: Flexible filter combinations for multi-target, multi-color fluorescence detection.
  3. Accurate & Stable Signal Acquisition: Synchronized detection enhances reliability and reduces errors.
  4. Optimized Operating Experience: Adjustable screen and touch interface for user comfort.
  5. Rich Analysis Tools: Comprehensive analysis modes for mainstream qPCR applications in research and clinical settings.
  6. Stable & Durable: Power failure protection and stand-alone operation safeguard experiments and reduce environmental dependency.

Working Principle

Utilizes high-efficiency semiconductor refrigeration for rapid, uniform temperature control during amplification. T-Optical top excitation and SSLPI static fluorescence CCD imaging ensure highly sensitive, synchronous signal acquisition for all wells—avoiding errors from mechanical scanning. Multi-group filter system enables precise multi-channel, multi-color detection. Control system supports power failure protection and independent offline operation for robust safety and stability.

Application Areas

  1. Clinical diagnosis: pathogen detection, genetic disease screening, tumor gene analysis.
  2. Basic research: gene expression, mutation detection, transgenic research.
  3. Drug development: target detection, gene function validation.
  4. Agricultural science: plant disease monitoring, germplasm identification.
  5. Environmental science: microorganism quantification in water/soil.
  6. Food safety: pathogen and GMO detection.
  7. Forensic science: DNA quantification, genotyping.
  8. Teaching & training: molecular biology experiments and technical education.
Technical Parameters
three-block real-time fluorescence quantitative pcr
ModelTC300
Block3 sets of 32 wells x 0.1 ml
Sample capacity5ul to 100ul
Supported tube typeswhite 0.1 ml PCR tubes, 8-strip tubes with optical flat caps
Control methodControlled by computer software or via the instrument built-in touchscreen
Optical systemSSLP short optical path static fluorescence CCD imaging technology
Display10.1" full-color TFT LCD touchscreen display, adjustable within 90 degrees
Temperature Control Range0°C to 105°C
Heating rate8°C per second
Cooling rate6°C per second
Temperature uniformity±0.2°C, at 90°C
Temperature accuracy±0.1°C, at 90°C
Temperature resolution0.1°C
Gradient range30°C to 100°C
Light sourcelong-life LED
Detectorhigh-sensitivity CCD
Dynamic detection range1 to ten billion
Sensitivity≥1 copy
Detectable fluorophore dyesF1: FAM|SYBR Green
F2: VIC|HEX|JOE|CY3|TET(customizable)
F3: ROX|TEXAS-RED|TAMRA(customizable)
F4: CY5|Quasar 670
F5: CY5.5
customizable
Fluorescence excitation wavelength400nm to 800nm
Fluorescence detection wavelength500nm to 800nm
Data export formatsTXT, PDF, Word, Excel
Power-off protectionyes
Communication interfacesEthernet, USB 2.0, supports data export via USB flash drive, wifi
Power supply100Vac to 240Vac, 50-60Hz, 1200W
Dimensions375x268x400mm
Weight16kg