thermal cycler with optional gradient and non-gradient

Modern thermal cycler is equipped with high-definition color touch screen, real-time dynamic display of experimental curves, users can always grasp the progress of the experiment, support a variety of reaction modules and program storage, is the core equipment necessary for life science laboratories.

Main Features of the Thermal Cycler

1. high-definition 7-inch TFT full-color touch screen: user-friendly interface, support for real-time monitoring of graphical curves, intuitive and convenient operation.
2. the temperature uniformity: to ensure that the temperature of the sample in each well is the same, to improve the experimental repeatability and amplification efficiency.
3. modular design, easy to replace: no need to use tools to replace the reaction module to meet different experimental needs.
4. large-capacity program storage: built-in storage of 10,000 experimental programs, through the USB interface to support unlimited program import and export.
5. Gradient and standard function free switching: suitable for different PCR experiments, convenient for primer optimization and condition screening.
6. compact and exquisite appearance design: light structure, beautiful shape, suitable for experimental table placement.
7. advanced heat dissipation system: forward and rear air outlet design, multiple instruments can be placed side by side, saving experimental space.
8. Diversified module configuration: support a variety of well plate specifications, including in situ carrier plate, 96-well × 0.2 ml, 77-well × 0.5 ml combination module, standard 96-well module and high-throughput 384-well module.
9. Intelligent hot cover control: when the program ends or the sample temperature drops below the set value, the hot cover closes automatically to protect the sample and prolong the life of the equipment.

Advantages

1. accurate temperature control, improve the success rate of the experiment: good temperature uniformity to ensure stable and reliable amplification effect.
2. easy to operate, improve work efficiency: the combination of touch screen and graphical interface allows users to easily set and monitor.
3. adaptable to meet diversified needs: multi-module support for a variety of reaction volumes and throughput, covering from routine PCR to high-throughput screening.
4. space utilization: reasonable heat dissipation design, multiple devices placed side by side without affecting performance.
5. Convenient data management: massive program storage and USB import/export function, which is convenient for standardization of experimental process and repeated use.

Working Principle

Thermal Cycler repeatedly cycles the temperature of the samples in the PCR reaction tubes or plates according to the set program by precisely controlling the heating element and the refrigeration system. Each cycle consists of:
1, denaturation phase: high temperatures, typically 94°C to 98°C, separate double-stranded DNA into single strands.
2, Annealing phase: reduced temperature, typically 50°C to 65°C to bind the primer to the target DNA sequence.
3, Extension phase: moderate temperature, usually 72°C allows the DNA polymerase to extend the new strand along the template.
The gradient function enables parallel screening of multiple conditions in the same experiment by setting different temperatures in different areas of the reaction plate, which improves experimental efficiency and optimization accuracy. The built-in temperature sensor provides real-time feedback of temperature data to ensure the precise execution of thermal cycling.

Application Areas

1. molecular biology research: DNA amplification, gene cloning, gene expression analysis.
2. Medical diagnosis: pathogen detection, genetic disease screening, tumor marker analysis.
3. Agricultural research: transgenic crop detection, germplasm identification.
4. Forensic identification: DNA fingerprint analysis.
5. Environmental monitoring: microbial pollution detection.
6. Teaching experiment: PCR technology training in life science related courses.
7. Pharmaceutical industry: gene-related experiments in the process of drug development.